Effect of koumine on the inflammation of RAW264.7 cells induced by LPS
10.16303/j.cnki.1005-4545.2017.08.22
- VernacularTitle:钩吻素子对LPS诱导RAW264.7细胞炎症的作用
- Author:
Zhihang YUAN
;
Hui YUAN
;
Jing WU
;
Xiaojun CHEN
;
Zhaoying LIU
;
Zhiliang SUN
- Keywords:
koumine;
lipopolysaccharide;
macrophagocyte;
inflammation
- From:
Chinese Journal of Veterinary Science
2017;37(8):1553-1557
- CountryChina
- Language:Chinese
-
Abstract:
To reveal the anti-inflammation mechanism of koumine,we determined effects of different concentrations of koumine(100,200,400 mg/L) on the secretion of NO,IL-1β,IL-6 and TNF-a by nitrate reductase and ELISA as well as the mRNA of iNOS,IL-1β,IL-6 and TNF-α.Western blot was used to detect iNOS protein expression.The results showed that 100,200,400 mg/L of koumine can significantly inhibit the secretion of NO,IL-1β,IL-6 and TNF-α of RAW264.7 cell (P＜0.01).Koumine can dose-dependently down-regulate the mRNA of iNOS,IL-1β,IL-6 and TNF-α,meanwhile koumine can also significantly inhibit the protein expression of iNOS.The results indicated that koumine may play an anti-inflammation activity by mean of the reduction of NO and mediator of inflammation,down-regulating the mRNA expression of iNOS,IL-1β,IL-6 and TNF-a,decreasing protein expressions of iNOS.
