Analysis of virulence genes and antibiotics resistance of Staphylococci isolated from mastitis cows in parts of Guangdong province

VernacularTitle:广东省部分地区奶牛乳房炎凝固酶阴性葡萄球菌的毒力基因和耐药性分析
Author: Yao SUN ; Kun JIA ; Xiaokun PING ; Liguo YUAN ; Shoujun LI ; Guihong ZHANG
Keywords: mastitis; coagulase negative Staphylococci; virulence; resistance
From: Chinese Journal of Veterinary Science 2017;37(8):1495-1500
CountryChina
Language:Chinese
Abstract: To evaluate the virulence genes and antibiotics resistance of Staphylococci species in mastitis cows isolated from parts of Guangdong,and then provide scientific basis of the prevention of bovine mastitis.Forty strains Staphylococci isolated from milk samples of 110 mastitis cows were collected,and virulence genes,resistance genes and disinfectant resistant genes were detected by PCR,and antibiotics resistance with K-B paper method.The results showed that the highest virulence gene was fnbp (17.5％),followed by seb (15％) and tsst (15％),and virulence genotype was complex.The most prevalent antibiotic resistance drug wvas streptomycin,followed by erythromycin and penicillin G,and CNS were susceptible to oxacillin,ceftraxone and cefazolin.The most prevalent antibiotic resistance genes was quinolones gene qnrA/B/C/D(20％),the resistance to streptomycin was mediated by aac6-aph2.The most prevalent disinfectant resistant gene was qacG (225 ％).The genotype of antibiotics resistance gene and disinfectant resistant gene was complex.