Lyophilized biological preparation of nematode-trapping fungus-Arthrobotrys oligospora
10.16303/j.cnki.1005-4545.2017.08.14
- VernacularTitle:捕食线虫性真菌-少孢节丛孢菌冻干生物制剂的制备
- Author:
Linjun CHEN
;
Xingduan LIU
;
Xiaoye YANG
;
Rui WANG
;
Wei ZHANG
- Keywords:
nematode-trapping fungus;
Arthrobotrys oligospora;
culture of spore;
lyophilized preparation
- From:
Chinese Journal of Veterinary Science
2017;37(8):1512-1516
- CountryChina
- Language:Chinese
-
Abstract:
In the study,nematode-trapping fungus-Arthrobotrys oligospora was firstly cultivated in Sabouraud dextrose broth medium containing 0.05％ of agar,then transferred to the corn meal agar medium.A.oligospora conidiospores was eluted from the media in different time and lyophilized after being counted,then the resuscitation of lyophilized spores was also observed,in oder to evaluate their nematicidal dosage and nematode-trapping efficacy in vitro.The results of the study were as follows:by observing the germination rate,growth rate and nematode-trapping rate of lyophilized spores from A.oligospora.The maximum germination rate of lyophilized A.oligospora conidiospores was 79.5％ on the 4 th day after inoculation,and the average growth rate was 3.4 mm/d;the maximum nematode-trapping rate was 95.8％ on the 7 th day after larvae were added on the media,and the average nematode-trapping rate was 74.0％.Compared with the control groups,the differences were both no significant (P＞0.1)in average growth and nematode-trapping rate.The results show that the freeze-dried preparation materials was accessible and simple,with good resuscitation.After further optimization it will display the prospect of industrialization application.
