The Killing Effects and Preliminary Mechanism of Natural Plant-derived Antimicrobial Solution(PAMs)to Human Liver Cancer HEPG-2 Cells
10.13241/j.cnki.pmb.2017.26.003
- VernacularTitle:一种天然植物抗菌液对HEPG-2细胞的杀伤及作用机制研究
- Author:
Jinyi QIU
;
Jiaming HUANG
;
Yijie ZHOU
;
Rongkun DOU
;
Zhenfei BI
;
Yalan YANG
;
Yinsong DONG
;
Danzhou XIANGFEI
;
Canquan MAO
- Keywords:
Natural plant-derived antimicrobial solution(PAMs);
Folk medicine;
HEPG-2 cancer cells;
Apoptosis pathway
- From:
Progress in Modern Biomedicine
2017;17(26):5011-5016
- CountryChina
- Language:Chinese
-
Abstract:
Objective:In this study,a series of experiments were conducted to research the mechanism of anticancer and preliminary molecular effects of PAMs on the HEPG-2 cancer cells.Methods:Morphological observation and MTT assay were used to explore the inhibition and killing effect of PAMs acting on HEPG-2.AO/EB staining and Annexin V-FITC/PI staining were employed to observe the apoptosis of HEPG-2 treated with PAMs.The expression level of Foxm1,bcl-2 and others genes in HEPG-2 cells were detected by using qRT-PCR and western blot.Wound healing and transwell experiments determined if PAMs can inhibit the migration of HEPG-2.Results:PAMs can inhibit and kill HEPG-2 cells in time and dose-dependent manners,and the cytotoxic effects were closely related to the cell apoptosis.The mRNA expression of foxm1,bcl-2 and surviving gene were remarkably decreased in HEPG-2 cells after the treatment of PAMs.PAMs decreased the FoXM1 protein expression in HEPG-2 cells,while up-regulating thep53 protein expression.,and it could also inhibit the migration of cancer cells.Conclusions:The possible molecular mechanism for the killing of HEPG-2 cancer cells by PAMs was proposed.By down-regulating the expression of foxm1 and up-regulating the expression of p53,the transcriptional expression of their downstream target genes survivin and bcl-2 was inhibited or reduced,hence enhancing the cancer cell apoptosis.This study provides an important foundation for the development of anti-cancer Chinese folk medicine based on PAMs.
