Construction of vibrio vaccine strain and expression of antigen gene:attenuated Listeria monocytogenes as vaccine vector

VernacularTitle:基于载体减毒单增李斯特菌的弧菌疫苗菌株构建
Author: Chengchao DING ; Guowei CHEN ; Manman XIE ; Liang GUO ; Jie LI ; Jingjuan SUN ; Qing LIU
Keywords: Attenuated Listeria monocytogenes; Vaccine vector; Vibrio vaccine; Ompk; Antigen gene
From: Chinese Journal of Immunology 2017;33(9):1281-1285,1290
CountryChina
Language:Chinese
Abstract: Objective:To provide a potential platform for transferring specific antigen against fish bacterial diseases based on attenuated Lm (EGDe-ΔactA/ΔinlB).Methods: Attenuated Lm (EGDe-ΔactA/ΔinlB) was used to express outer membrane protein K (Ompk),a conserved and effective vaccine candidate in vibrio.The identification of recombinant strains and detection of antigen genes were operated with PCR and RT-PCR,respectively.Results: The results of PCR showed that Lm-Ompk (L-O),Lm-Lmo0576-Ompk (L-L-O) and Lm-P-Ompk (L-P-O) were constructed successfully.The identity of foreign gene was 100％ compared with sequence of NCBI.The analysis of transcription showed that the expressions of Ompk in L-O,L-L-O and L-P-O were significant (P<0.001).Moreover,the expression of Ompk in the condition of antibiotic was higher than that in the BHI without antibiotic (P<0.05).Conclusion: Lm-Ompk (L-O),Lm-Lmo0576-Ompk (L-L-O) and Lm-P-Ompk (L-P-O) were constructed successfully.