Effect of TRAP1 on invasion and migration of human bladder cancer through TGF/Smad3 signal pathway
10.3969/j.issn.1000-484X.2017.09.006
- VernacularTitle:TRAP1通过TGF/Smad3通路对膀胱癌的迁移和侵袭能力的影响
- Author:
Zhe WANG
;
Jing ZHANG
;
Huaian CHEN
;
Chao ZHANG
;
Xiaoyun ZHANG
;
Shuo LIU
;
Wenlong MIAO
;
Fengqi LI
- Keywords:
TRAP1;
Bladder cancer;
TGF;
Smad3;
ROS
- From:
Chinese Journal of Immunology
2017;33(9):1306-1309,1314
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect and related mechanism of TRAP1 on the invasion and migration of human bladder cancer through TGF/Smad3 signal path.Methods: Selected from BIU-87 of high expression of TRAP1 in bladder cancer cell lines through Western blot techniques.TRAP1 knockdown lentivirus (LV3-TRAP1) was used to silence the expression of TRAP1.GFP fluorescene and PCR detector was used to detected the efficiency of gene silencing and the effectiveness of gene silencing;effect of TRAP1 on the invasion and migration ability of BIU-87 were detected by Transwell matrigel invasion assays and wound healing assays,CM-H2DCFDA fluorescent staining was used to deteced the cell ROS of BIU-87 with LV3-TRAP1.Detected the level of TGF/Smad3 signal protein by Western blot.Results: LV3-TRAP1 lentivirus could effectively inhibit the expression of TRAP1 compared with LV3-NC.LV3-TRAP1 lentivirus could effectively inhibit the cell RPS of BIU-87.Knockdown the expression of TRAP1 could inhibit the invasion and migration of BIU-87.Knockdown the expression of TRAP1 in BIU-87 could reduce the protein level of TGF/Smad3.Conclusion: Silencing TRAP1 could inhibit the invasion and migration of bladder cancer cell through TGF/Smad3 signal pathway.
