Influence of specific siRNA silencing survivin gene on proliferation and invasion of human tongue cancer Tca8113 cells in vitro
10.3969/j.issn.1000-484X.2017.09.007
- VernacularTitle:特异性干扰survivin基因表达对人舌癌Tca8113细胞体外增殖和侵袭性的影响
- Author:
Runhong MU
;
Fang MA
;
Yongchao LIU
;
Xiangming WANG
- Keywords:
survivin;
siRNA;
Proliferation;
Apoptosis;
Migration
- From:
Chinese Journal of Immunology
2017;33(9):1310-1314
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of siRNA interfering survivin gene on proliferation of human tongue cancer Tca8113 cells,and clarify the effect of survivin Tca8113 cells biomechanism.Methods: The Tca8113 cells at logarithmic growth phase were selected and divided into interference group,negative control group,and blank control group.The relative levels of survivin mRNA and survivin protein expression of 3 groups were detected by RT-PCR and Western blot assay,the inhibitory rate of proliferation of Tca8113 cells was checked by MTT method,the apoptotic rate was assessed by flow cytometry(FCM),the the migration ability of Tca8113 cells were detected by Wound healing assay.Results: Compared with control group,the survivin mRNA and protein expression was markedly down-regulated in Tca8113 cells following RNA interference treatment(P<0.05),the cell proliferation were down-regulated in interference group(P<0.05),the cell apoptotic rate were up-regulated in interference group(P<0.05),the migration ability was significantly decreased in interference group(P<0.05).Conclusion: The expression of siRNA-survivin can significantly inhibit the invasion in tongue cancer Tca8113 cells,indicating it might be a potential biological therapeutic target for tongue cancer.
