Effect of metformin on proliferation,cell cycle and apoptosis of U937 cells
10.3969/j.issn.1000-484X.2017.09.008
- VernacularTitle:二甲双胍对U937细胞增殖、周期及凋亡的影响
- Author:
Junru LI
;
Huifang LI
;
Jia ZHOU
;
Liyun ZHANG
;
Xiao LU
;
Daming ZUO
;
Zhengliang CHEN
- Keywords:
Metformin;
U937;
Proliferation;
Cell cycle;
Apoptosis;
AMPK/p53
- From:
Chinese Journal of Immunology
2017;33(9):1315-1319
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of metformin on proliferation,cell cycle and apoptosis of U937 cells.Methods: U937 cells were treated with different concentrations of metformin,collected cells in 24,48 and 72 hours.Subsequently,cell proliferation was assessed by CCK-8 assay,and the cell cycle and apoptosis were analyzed by flow cytometry (FCM).The expression of Bcl-2,Bax,p-AMPK,p53 were determined by Western blot.Results: The proliferation of U937 cells was inhibited by metformin in a time-and dose-dependent manner.Metformin-treated cells were arrested at G0/G1 phase,the cell frequency at G0/G1 phase was increased in a time-and dose-dependent manner.Metformin also induced cell apoptosis in a dose-dependent manner.It showed that 20 mmol/L metformin induced cell apoptosis in a time-dependent manner.The expression of p-AMPK,p53,Bax was up-regulated while Bcl-2 expression was down-regulated after metformin treatment.Conclusion: Metformin could inhibit the U937 cell proliferation,block the cell cycle at G0/G1 phase,and induce cell apoptosis,which may partially be attribute to the up-regulation of Bax,down-regulation of Bcl-2,activation of AMPK/p53 signaling.
