Effect of RNA Binding Protein of QKI-5 on Breast Cancer Cell MCF-7 of Proliferation
10.13241/j.cnki.pmb.2017.25.004
- VernacularTitle:RNA结合蛋白QKI-5对乳腺癌细胞MCF-7增殖的影响研究
- Author:
Yi ZHAO
;
Qingli ZHAO
;
Ji MA
;
Qian CAI
;
Geng ZHANG
- Keywords:
QKI-5;
Breast cancer;
Western blot;
MTT assay
- From:
Progress in Modern Biomedicine
2017;17(25):4816-4819
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To check RNA binding protein QKI-5's expression level in breast cancer cells and inhibiting effect on cancer cell proliferation.Methods:QKI-5's expression level was checked in different breast cancer cells by Western blotting,cancer cells of overexpressed QKI-5 gene could be stabilized by slow virus infection construction,MTT and FCM were used to check cell period to express QKI-5's influence on cell proliferation and period.Results:MCF-7 cells have relatively low QKI-5 expression level in three breast cancer cells,MTT experiment result has obvious reduced influence on QKI-5's expression of MCF-7 cell proliferation P＜0.05,mean-while,cell period inspection shows that overexpressed QKI-5's MCF-7 cells have obvious G1 retardant,cells in S and G2/M periods are reduced.Conclusion:High expression of QKI-5'in breast cancer could cause slow cell proliferation by inhibiting cancer cell period,hence causing limited tumor growth.
