Effects of miR-218 on SNX4 protein on proliferation and invasion of breast cancer cells
10.3969/j.issn.1000-484X.2017.09.009
- VernacularTitle:miR-218调控SNX4蛋白对乳腺癌细胞增殖和侵袭的影响
- Author:
Jianbo ZHANG
;
Wei SONG
;
Yuanyuan WANG
;
Mingge LIU
;
Miaomiao SUN
- Keywords:
Breast cancer;
miR-218;
SNX4;
Invasion
- From:
Chinese Journal of Immunology
2017;33(9):1320-1325
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of miR-218 on the proliferation and invasion of breast cancer cells.Methods: The expression of miR-218 in breast cancer tissues and breast cancer cell lines was detected by qPCR.The relationship between the expression of miR-218 and the clinicopathological parameters of breast cancer were analyzed.Double luciferase assay was used to detect the relationship between miR-218 and SNX4.MTT assay and invasion assay were used to detect the proliferation and invasion of breast cancer cells after overexpression of miR-218.MTX assay and invasion assay were used to detect the recovery level of SNX4 on the proliferation and invasion of breast cancer cells.The effect of miR-218 on the tumorigenesis of breast cancer cell lines was examined by tumorigenesis in nude mice.Results: The expression level of miR-218 in breast cancer tissue and MCF-7 cell line was higher.The expression of miR-218 was associated with pathological stage of breast cancer and lymph node metastasis.SNX4 may be the target of miR-218.Overexpression of miR-21 could inhibit the proliferation and invasion of breast cancer cells.Overexpression of SNX4 could reverse the inhibitory effect of miR-218 on breast cancer cells.Overexpression of miR-218 could inhibit the breast cancer cell line in nude mice tumorigenic ability.Conclusion: miR-218 can up-regulate the expression of miR-218 in breast cancer,and miR-218 can regulate the expression of SNX4 in breast cancer cell proliferation and invasion.
