Effect of culture supernatant of rat bone mesenchymal stem cells on biological characteristics of RSC96 cells
10.3969/j.issn.1000-484X.2017.09.003
- VernacularTitle:大鼠骨髓间充质干细胞培养上清液对RSC96细胞生物学功能的影响
- Author:
Yongbin MA
;
Suohua GE
;
Xuefeng WANG
;
Jianhua SU
- Keywords:
Bone marrow mesenchymal stem cells;
RSC96 cells;
Proliferation;
Migration
- From:
Chinese Journal of Immunology
2017;33(9):1291-1295
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of culture supernatant of rat bone marrow mesenchymal stem cells (BMSCs) on biological characteristics of RSC96 Schwann cells.Methods: BMSCs of Sprague Dawley rat were cultured and purified in vitro.The BMSCs culture supernatant medium (BMSC-CM) was collected from the third generation of BMSCs.Using MTT method to detect proliferation influence of BMSC-CM on RSC96 cells,and using plate cloning assay to analysis a single cell proliferation effect.The migration analysis of BMSC-CM on RSC96 cells were detected by scratch and TranswellTM chamber assay,Western blot tested Bax and Bcl-2 proteins changes in RSC96 cells.Results: The third generation of BMSCs was long spindle-shaped and vortex-like;the result of Osteogenesis staining was positive;lipid droplets appeared after adipogenesis induction.BMSC-CM inhibited the proliferation and migration of RSC96 cells.Meanwhile,Bcl-2 protein decreased and Bax protein increased in RSC96 cells after BMSC-CM treatment.Conclusion: BMSC-CM can promote apoptosis and inhibit the proliferation and migration of RSC96 cells.
