Expression of SHIP 1 in the Patients with Acute Myeloid Leukemia and Its Influence on the Apoptosis of Human Leukemia Cells
10.13241/j.cnki.pmb.2017.23.009
- VernacularTitle:SHIP1在急性髓细胞白血病患者中的表达及对人白血病细胞凋亡的影响研究
- Author:
Xiaorui WANG
;
Wenqian LI
;
Jianming FENG
;
Kuo SHEN
;
Guo AI
;
Guoxiong HAN
;
Yi MENG
- Keywords:
Acute myeloid leukemia;
SHIP1;
Human leukemia cells;
Apoptosis
- From:
Progress in Modern Biomedicine
2017;17(23):4441-4445
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of SHIP1 in the patients with acute myeloid leukemia and its effect on the apoptosis of human leukemia cells.Methods:The expression of SHIP1 in the bone marrow of patients with acute myeloid leukemia was detected by Westem blot.U937 cells was transfected with SHIP1 expression vector (pEGFP-SHIP1 group) and empty vector control (pEGFP group) respectively,U937 cells without transfection were used as the control group.Flow cytometry was used to detect the apoptosis of the cells,the expression of SHIP1,Bcl-2,Bax,Akt,p-Akt were detected by western blot.Results:The expression of SHIP1 in the bone marrow of patients with acute myeloid leukemia was significantly lower than that of the normal human bone marrow SHIP 1 (P＜0.01).The SHIP1 and Bax expressions as well as the apoptotic rate ofpEGFP-SHIP1 group were significantly higher than those of the control group(P＜0.01),while the Bcl-2 and p-Akt expressions were significantly lower than those in the control group(P＜0.01).Conclusions:SH-P1 expression was down regulated in the bone marrow of patients with acute myeloid leukemia.SHIP1 could promote the apoptosis of human leukemia cells via Akt signaling pathway.
