Function of lncRNA TUG1 in Insulin Secretion from Pancreatic Beta Cells
10.13241/j.cnki.pmb.2017.25.011
- VernacularTitle:lncRNA TUG1在胰岛β细胞分泌胰岛素中的功能研究
- Author:
Lihua CAO
;
Dandan YIN
;
Chengcai XIA
;
Ning WANG
;
Wei DE
- Keywords:
LncRNA TUG1;
β cells;
Insulin secretion
- From:
Progress in Modern Biomedicine
2017;17(25):4847-4851
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the role of lncRNA TUG1 in pancreatic β cells functioning both in vitro and in vivo.Methods:The lncRNA TUG1 expression in mice pancreas,brain,muscle and other different tissues was examined through qRT-PCR.MTT,flow cytometry,GSIS,ELISA and immunochemistry analyses were performed to detect the effect of lncRNA TUG1 on insulin secretion in vitro and in vivo.Results:lncRNA TUG1 was highly expressed in pancreatic tissue compared with other organ tissues.Knockdown of lncRNA TUG1 expression resulted in decreased insulin secretion in β cells both in vitro and in vivo.Immunochemistry analyses showed decreased relative islet area after treatment with lncRNA TUG1 siRNA.Conclusions:Downregulation of lncRNA TUG1 expression can affect insulin secretion in pancreatic β cells in vitro and in vivo,and lncRNA TUG1 may represent a factor that regulates the function of pancreatic β cells.
