Exploration of enzyme-MnO2 nanosheets hybridization probe for sensitively colorimetric self-indicating of glucose
10.11665/j.issn.1000-5048.20170410
- VernacularTitle:酶-二氧化锰纳米片杂化探针自指示比色传感葡萄糖
- Author:
Li LI
;
Fengguo XU
;
Jinlong CHEN
- Keywords:
glucose;
sensor;
colorimetry;
MnO2;
nanosheets;
probe;
glucose oxidase
- From:
Journal of China Pharmaceutical University
2017;48(4):453-460
- CountryChina
- Language:Chinese
-
Abstract:
A colorimetric self-indicating probe for glucose was constructed by self-assembly of MnO2 nanosheets (MnO2 NSs) and glucose oxidase(GOD) in this paper.Under the weak acidic medium,glucose oxidase specifically catalyzes glucose into gluconic acid and hydrogen peroxide.The by-product of hydrogen peroxide could efficiently dissolve the MnO2 nanosheets,resulting into a significant decrease of the characteristic absorbance at 374 nm assigned to MnO2 NSs.Furthermore,the absorbance difference was linearly proportional to the concentration of glucose ranging from 1 to 20 μmol/L The fitted curve could be used for quantification of glucose with a correlation coefficient of 0.990 1.And the detection limit as low as 0.1 μmol/L could be reached based on the definition of three times of the deviation of the blank signal (3σ) and there was negligible interference with other co-existing amino acids,anions,cations and protein,which indicated high sensitivity and selectivity of the hybrid probe.The construction strategy of designated probe is readily generalized in principle for detection of numerous analytes in view of reactive property of MnO2 and the diversity of enzymes.
