Anti-liver fibrosis activities of human insulin-like growth factor-1 in vitro
10.11665/j.issn.1000-5048.20170413
- VernacularTitle:人胰岛素样生长因子-1的体外抗肝纤维化活性
- Author:
Aini WAN
;
Dongsheng XU
;
Yanfei CAI
;
Yun CHEN
;
Jian JIN
;
Huazhong LI
- Keywords:
insulin-like growth factor-1;
transforming growth factor β1;
liver cells;
hepatic stellate cells;
liver fibrosis
- From:
Journal of China Pharmaceutical University
2017;48(4):476-482
- CountryChina
- Language:Chinese
-
Abstract:
This study was focus on investigating the anti-liver fibrosis effects of insulin-like growth factor-1 (IGF-1) in vitro.The effects of IGF-1 on human liver L-02 cell viability and cell cycle were observed.CC14-induced L-02 cell injury was set up to detect the anti-apoptotic activity of insulin-like growth factor-1 (IGF-1).Transforming growth factor β1 (TGF-β1) induced hepatic stellate cell line (HSC-T6) were used as a liver fibrosis model in vitro to analyze the effects of IGF-1 on the expression of liver fibrosis proteins and intracellular TGF-β1/Smad signaling pathway in HSC-T6 cells.The results showed that IGF-1 could relieve the growth inhibition effects of TGF-β1 on L-02 cells,increase the viability of L-02 cells injured by CCl4,decrease the expression of liver fibrosis proteins,and inhibit the TGF-β1/Smad signaling pathway by inhibiting the phosphorylation of Smad3.Our study suggested that IGF-1 exerted anti-liver fibrosis effects by stimulating L-02 cells proliferation,reducing cell damage and inhibiting ECM accumulation via interfering TGF-β1/Smad signaling pathway.
