Protective effects of Cordyceps sinensis polysaccharide CPS-A on angiotensin Ⅱ-induced injury of liver L02 cells
10.11665/j.issn.1000-5048.20170415
- VernacularTitle:虫草多糖CPS-A对血管紧张素Ⅱ诱导肝L02细胞损伤的保护作用
- Author:
Zhengqi ZHA
;
Qiuli CHEN
;
Yufeng WANG
;
Hui ZHU
;
Ying WANG
;
Hongping YIN
;
Long YUE
- Keywords:
Cordyceps sinensis polysaccharide-A (CPS-A);
L02 cells;
angiotensin Ⅱ;
inflammation
- From:
Journal of China Pharmaceutical University
2017;48(4):490-495
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed at the molecular mechanism of Cordyceps sinensis polysaccharide-A(CPS-A) on angiotensin (Ang Ⅱ)-induced injury of L02 cells.The effect of AngⅡ and CPS-A on the proliferation of L02 cells was analyzed by MTT assay.PCR,Real-Time PCR and Western blot were also employed to determine the expression of IL-1β,AT1R,AT2R,NF-κB p65,TNFα and other inflammatory factors at mRNA and protein levels.The results showed that Ang Ⅱ and CPS-A could inhibit the proliferation of L02 cells by 1 × 10-5 mol/L and 200 μg/ mL,respectively.PCR,Real-Time PCR and Western blot showed that CPS-A could significantly down-regulate IL-1 β,TNF-α,NF-κB and AT1R.CPS-A has a good protective effect on AngⅡ-induced L02 cell injury.
