Solid-phase screening of antibodies in blood and its effect on clinical blood transfusion
10.13303/j.cjbt.issn.1004-549x.2017.07.033
- VernacularTitle:血液中同种抗体的固相化筛查及其对临床输血效果的影响
- Author:
Qixiu YANG
;
Shasha HAN
;
Ying YANG
;
Jiamin ZHANG
;
Wei YAN
;
Ziyan ZHU
- Keywords:
solid-phase detection;
antibodies screening;
antibodies against blood cells;
adverse reactions of blood transfusion
- From:
Chinese Journal of Blood Transfusion
2017;30(7):748-751
- CountryChina
- Language:Chinese
-
Abstract:
Objective Detecting antibodies against RBCs,platelets,lymphocytes,and neutrophils by a solid-phase antibodies screening system.Methods mono-layer blood cell immobilized in the bottom of U-microplate respectively to prepare the solid-phase antibody screening system.Then detecting the antibodies exist or not in 2 150 random blood donor and 440transfusion patients' samples.Analyze the influence of antibodies against blood cells to the transfusion effect.Results 53RBC antibodies,22 platelet antibodies,13 lymphocyte antibodies,55 neutrophil antibodies were detected in random blood donor samples;31 RBC antibodies,38 platelet antibodies,24 lymphocyte antibodies,43 neutrophil antibodies and 115 mixed (two or more antibodies against RBC,platelets,lymphocytes or neutrophils exist at the same time) were detected in transfusion patients' samples after detection.233 suspected adverse reaction happened after transfusion,which antibodies were detected in 133 samples.Conclusion The antibodies against blood cells solid-phase screening system can applied in pre-and post-transfusion detection.The solid-phase screening method is more sensitivity than serologic tube test and flow cytometry.Existing of antibodies influence the transfusion effect.
