Simultaneous Determination of Epimedium Glycoside and Other 4 Kinds of Active Ingredients in Xianling Gubao Capsules by Three Wavelength Switching Method
10.3969/j.issn.1005-5304.2017.10.016
- VernacularTitle:三波长切换法同时测定仙灵骨葆胶囊中淫羊藿苷等5种活性成分含量
- Author:
Liang FENG
;
Shuhao CHU
;
Xiaocai WANG
- Keywords:
Xianling Gubao Capsules;
icariin;
asperosaponin Ⅵ;
psoralen;
angelicin;
epimedin C
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(10):68-71
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the method for simultaneous determination of epimedium glycoside and other 4 kinds of active ingredients in Xianling Gubao Capsules by three wavelength switching method. Methods An Waters Atlantis T3 C18 column (4.6 mm × 250 mm, 5 μm) was used with the mixture of acetonitrile-0.05％ formic acid solution as the mobile phase in gradient elution (0–5 min, 12％–20％ A; 5–15 min, 20％–55％ A; 15–35 min, 55％ A;35–55 min, 55％–76％ A; 55.1 min, 12％ A; 55.1–60 min, 12％ A). Detection wavelength was as follow: 0–30 min, 212 nm; 30–42 min, 246 nm; 42–60 min, 270 nm. The flow rate was 1.0 mL/min. The column temperature was 30 ℃. Results The calibration curves of asperosaponin Ⅵ, psoralen, angelicin, epimedin C, and icariin were in good linearity among the ranges of 101.6–3048 ng, 8.7–261 ng, 7.9–237 ng, 117.2–3516 ng, 78–2340 ng, respectively. In the instrument precision test, stability test, repeatability test, the RSD was less than 3％. The average recoveries were 99.83％, 100.35％, 100.59％, 100.60％, 99.72％, respectively, and all the RSD were less than 3％. Conclusion The method is sensitive, accurate, and separation effect is good, which can provide a basis for quality evaluation standard of Xianling Gubao Capsules.
