Simultaneous Determination of Contents of 6 Components in Aerial Part of Rheum tanguticum Maxim.ex Balf by HPLC Method
10.3969/j.issn.1005-5304.2017.10.018
- VernacularTitle:HPLC同时测定唐古特大黄地上部分6种化学成分含量
- Author:
Liping CHEN
;
Maoxing LI
;
Xingfu MA
- Keywords:
Rheum tanguticum Maxim.ex Balf;
chrysophanol;
emodin;
physcion;
aloe-emodin;
sennoside A;
sennoside B;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(10):76-80
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an HPLC method to determine the contents of chrysophanol, emodin, physcion, aloe-emodin, sennoside A, and sennoside B simultaneously in the aerial part of Rheum tanguticum Maxim.ex Balf. Methods The analysis was achieved with an Agilent ZORBAX SB-C18 analytic column (250 mm × 4.6 mm, 5 μm) by gradient elution of methanol-0.1％ phosphoric acid in water gradient elution system (0 min, 70％ B; 5 min, 65％ B;8–16 min, 60％ B; 18–23 min, 55％ B; 25–30 min, 45％ B; 32–39 min, 35％ B; 49–57 min, 24％ B; 65–72 min, 20％ B). The flow rate was 1.0 mL/min; detection wavelength was 254 nm; the column temperature was room temperature; the quantification used external standard method. Results The peak areas and concentrations of chrysophanol, emodin, physcion, aloe-emodin, sennoside A, and sennoside B showed good linear relationship within a certain concentration range (r≥0.9995); the RSD of precision was 0.75％–1.17％; the RSD of repeatability was 0.99％–2.06％; the RSD of stability was 0.97％–1.76％; the average recoveries were 99.7％–100.4％. The results showed that there were differences in content between the root and aerial part of Rheum tanguticum Maxim.ex Balf. Conclusion HPLC method for simultaneous determination of contents of 6 contents of the aerial part of Rheum tanguticum Maxim.ex Balf can be used as the references for quality control.
