Research on Component Determination of Tang-Bi-Kang Granules in Rat Serum by UPLC-ESI-MSn Method
10.11842/wst.2017.05.012
- VernacularTitle:UPLC-ESI-MSn法测定糖痹康颗粒入血成分研究
- Author:
Dongchao WANG
;
Ying WEI
;
Jiaqi GAO
;
Yibing LIU
;
Lingxia QU
;
Yongqiao LIU
;
Tunhai XU
;
Tonghua LIU
- Keywords:
Tang-Bi-Kang granules;
UPLC-ESI-MSn;
components in serum;
pharmaceutical material basis
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2017;19(5):774-779
- CountryChina
- Language:Chinese
-
Abstract:
Through comprehensively characterizing components in blood after oral administration of Tang-Bi-Kang (TBK) granules by UPLC-ESI-MSn,this study was aimed to explain the pharmaceutical material basis of TBK initially.UPLC-LTQ-Orbitrap was used under both positive and negative ion modes of electrospray ionization.The blank serum and rat serum after oral administration of TBK were analyzed.Components in rat serum were identified and characterized based on ion fragment information,evenelectron law,nitrogen rule and so on.Reference data was used to establish the UPLC-ESI-MSn method.The results showed that after oral administration of TBK granules,15 components were detected in the serum,of which 13 components were taken as the prototype to blood and 2 metabolites.It was concluded that constituents of TBK granules in rat serum were generated from compatibility of all herbal medicines.In rat serum,most of the components had been absorbed by rat's metabolism;a few were absorbed as the prototype.This research provided references for pharmacodynamic material basis and metabolism of TBL granules in vivo.
