The effects of low intensity pulsed ultrasound on the osteogenetic differentiation of BMSCs on titanium with different surface topography
10.3969/j.issn.1001-3733.2017.04.001
- VernacularTitle:低强度脉冲超声对不同钛表面骨髓间充干细胞成骨分化的影响
- Author:
Yan SONG
;
Gaoyi WU
;
Jing WANG
;
Lei CHEN
;
Xiaoyuan DU
;
Xiaotao XING
;
Jiaojiao ZOU
;
Guoxiong ZHU
- Keywords:
Low intensity Pulse ultrasound(LIPUS);
Bone marrow mesenchymal stem cells(BMSCs);
Osteogenetic differentiation;
Titanium surface
- From:
Journal of Practical Stomatology
2017;33(4):431-436
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of low intensity pulsed ultrasound(LIPUS) on the osteogenic differentiation of rat bone marrow mesenchymal stem cells(BMSCs) on titanium surface.Methods:BMSCs from Wistar rat bone marrow were respectively cultured on the flat titanium surface and the large grain blast acid etched(SLA) titanium surface,and induced by mineralization medium.Then,the cells were interfered by LIPUS and a control condition.Alkaline phosphatase(ALP) were quantitative determinated after 3 and 7 d mineralization induction respectively,ALP staining were observed after 14 d induction.Alizarin red staining were observed after 21 d mineralization induction.Osteogenic related protein and gene expressions were detected after mineralization induction.Results:ALP in culture medium of LIPUS group was higher than that of the control group after 3 d and 7 d mineralization induction(P＜0.05).LIPUS group showed stronger ALP staining and alizarin staining,and more mineralized nodules than control group.The expression of osteogenic related proteins,including Runx2,BMP2,OPN in LIPUS group increased.Osteogenic related genes expression,including ALP,Runx2,BMP2,OPN,OCN and Col-1 of the LIPUS group increased.Conclusion:The osteogenic differentiation of BMSCs on the fiat titanium surface or SLA titanium surface can be promoted by LIPUS.
