Resveratrol down-regulates acute pulmonarythromboembolism-induced pulmonary artery hypertensionand monocyte chemoattractant protein-1 in rats
10.3969/j.issn.1001-1978.2017.10.021
- VernacularTitle:白藜芦醇调控大鼠急性肺栓塞后肺动脉高压及MCP-1表达的机制研究
- Author:
Chun CHEN
;
Jianwei LIN
;
Guoping LI
;
Zhuochao REN
;
Yaqing LI
;
Jianping YAN
;
Liangxing WANG
- Keywords:
resveratrol;
acute pulmonary thromboembolism;
pulmonary artery hypertension;
monocyte chemoattraetantprotein-1;
inflammatory factor;
inflammatory response
- From:
Chinese Pharmacological Bulletin
2017;33(10):1436-1441
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the relationship between monocyte chemoattractant protein-1(MCP-1) and pulmonary artery hypertension after acute pulmonary thromboembolism(PTE), and to explore the effects and mechanisms of resveratrol with MCP-1 in the acute PTE as well.Methods The acute PTE model of Sprague-Dawley rats was replicated using self-thrombosis.The rats were randomly divided into five groups(Normal, Solvent, acute PTE, antibody Cl142, and resveratrol), and 1h, 4h, 8h and 3 points were observed in each group.A model of acute PTE was established by infusion of an autologous blood clot into the pulmonary artery through a polyethylene catheter.Resveratrol or Cl142, dissolved in 1％ dimethyl sulfoxide(DMSO), was administered to the animals through caudalvein 1 h prior to the beginning of acute PTE modeling.Rats in normal control group and solvent control group were injected with normal saline and 1％ DMSO respectively.The mean pulmonary artery pressure(MPAP) and the mRNA and protein expression of MCP-1 were measured at each time point.Results ① The acute PTE group MPAP, MCP-1 mRNA and protein expression were significantly higher than those of the control group(P<0.05) at the same time;② The resveratrol group′s MPAP and MCP-1 mRNA, protein expression were significantly lower than those of the acute PTE group(P<0.05) at the same time;③ The Cl142 group MPAP and MCP-1 mRNA, protein expression were markedly reduced in the acute PTE group(P<0.05) at the same time.Conclusions The large expression of MCP-1 after acute PTE is involved in the formation of pulmonary hypertension after acute PTE.Resveratrol can reduce the pressure of pulmonary artery after acute PTE by down-regulating the MCP-1 expression.
