Research on the Induced Differentiation of Adipose Derived Stem Cells and Its Origin
10.13241/j.cnki.pmb.2017.24.002
- VernacularTitle:脂肪干细胞的诱导分化及其来源的研究
- Author:
Sanhu WANG
;
Rongyong MAN
;
Xiqiang GAO
;
Changshun YANG
;
Huan DENG
;
Lin ZHU
;
Xiaofei ZHENG
;
Xiguo ZHOU
- Keywords:
Adipose-derived stem cells;
Adipose tissue;
Adipogenesis differentiation;
Osteogenesis differentiation;
Origin of adipose-derived stem cells
- From:
Progress in Modern Biomedicine
2017;17(24):4606-4609
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the induction and differentiation potential of ADSCs by tissue culture method,and to preliminary study on the origin of ADSCs.Methods:Using adipose tissue culture method to culture human ADSCs.The third generation of ADSCs for the adipogenic and osteogenesis differentiation,and staining by oil red O and alizarin red S.HE staining was performed after the seventh day culture of adipose tissue.Results:The primary human ADSCs were successfully cultured with adipose tissue culture method.ADSCs cultured to the eighth generation,still maintained a good proliferation ability and cell morphology.ADSCs can be successfully induced into adipose cells and bone cells.ADSCs were mainly distributed around the mesenchymal vascular and connective tissue,by HE staining of adipose tissue after seven days of culture.Conclusion:The cells that were cultured with adipose tissue have the potential to adipogenic and osteogenesis differentiation.The ADSCs were mainly distributed around the mesenchymal vascular and connective tissue.
