Influence of Chaihu Jiedu Decoction on LX-2 Cell Proliferation and Apoptosis
10.13241/j.cnki.pmb.2017.24.003
- VernacularTitle:柴胡解毒汤对人肝星状细胞LX-2增殖及凋亡的影响
- Author:
Jueyun WANG
;
Congyan TIAN
;
Jinqiao ZOU
;
Gongze ZHAO
;
Xinjie BAI
;
Xianzhao YANG
- Keywords:
Chaihu Jiedu decoction;
Human hepatic stellate cells;
Cell proliferation;
Apoptosis;
Cell experiment
- From:
Progress in Modern Biomedicine
2017;17(24):4610-4614,4625
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of Chaihu Jiedu decoction on human hepatic stellate LX-2 cells,and to explore the potential molecular mechanisms.Methods:The wistar rats were divided into the experimental group and the control group,respectively with Chaihu Jiedudecoction and saline lavage,then centrifugal and get the drug-containing serum and control serum.At the same time,trsuscitate cells.When we got the expected number of cells,we divided into the experimental group and the control group.The human hepatic stellate cells (LX-2) were with the drug serum for 24 h,36 h,48 h,72 h.Then the cell proliferation inhibition rate was measured by CCK-8,the apoptosis were detected by flow cytometry (Annexin V-FITC,PI staining method).Results:ChaihuJiedu decoction could inhibit proliferation of LX-2 cells 24 hours after dosing.The in hibition rate in 36 h,48 h,72 h were 0.37 ％,0.46 ％,0.44 ％ respectively.It could prevent LX-2 cells into proliferation and induce the apoptosis of LX-2 cells.The apoptosis rates in 48 h,72 h were (9.80±0.95)％,(36.40± 5.09)％ respectively,and there are difference of statistical significance(P＜0.05).Conclusion:Chaihu Jiedu decoction can inhibit the proliferation of LX-2 cells proliferation,and induce apoptosis,so as to interfere with course of the liver fibrosis.
