Inhibiting effects of gambogenic acid in combination withmiR-218 on cervival cancer HeLa cells and its mechanisms
10.3969/j.issn.1001-1978.2017.10.015
- VernacularTitle:miR-218对新藤黄酸抑制人宫颈癌HeLa细胞增殖作用的影响及机制研究
- Author:
Yurong SHI
;
Jian LIU
;
Sulian CHEN
;
Ying YANG
- Keywords:
gambogenic acid;
miR-218;
HeLa cells;
proliferation;
apoptosis;
Bcl-2/ Bax
- From:
Chinese Pharmacological Bulletin
2017;33(10):1405-1409
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the inhibitory effects of gambogenic acid in combination with miR-218 on cervical cancer HeLa cells and its mechanisms.Methods Eukaryotic expression vector of miR-218(pmi8-218) was transfected into HeLa cells.Transcript levels of miR-218 were quantified by real-time quantitative PCR.HeLa cells were incubated with different concentrations of gambogenic acid alone or in combination with pmiR-218.The cell growth inhibiting ratio of HeLa cells was assessed by MTT assay.Cell apoptosis was measured by fluorescence activated cell sorting.The expression levels of Bcl-2, Bax and E-cadherin were measured by Western blot and qRT-PCR.Results Levels of miR-218 transcript significantly increased in pmiR-218 transfected HeLa cells.Overexpression of miR-218 may enhance the sensitivity of HeLa cells to gambogenic acid.Over expression of miR-218 could enhance the effect of gambogenic acid on inhibition cell proliferation, promoting apoptosis of HeLa cells.pmiR-218 could enhance the regulation of Bax expression and decrease the expression of Bcl-2 in HeLa cells.Conclusions Over expression of miR-218 may enhance the sensitivity of HeLa cells to gambogenic acid;miR-218 can enhance the effect of gambogenic acid on inhibition cell proliferation and promote the apoptosis of HeLa cells, and the mechanism may be related to down-regulation of Bcl-2/Bax expression.
