SAHA affects cell cycle of MCF-7 breast cancer cells byregulating acetylated levels of p21WAF1/CIP1 promoter
10.3969/j.issn.1001-1978.2017.10.018
- VernacularTitle:组蛋白去乙酰化酶抑制剂调控p21WAF1/CIP1启动子乙酰化水平影响乳腺癌MCF-7细胞周期
- Author:
Hui ZHOU
;
Weiqiang ZHOU
- Keywords:
breast cancer;
estrogen receptor positive cell line;
MCF-7;
SAHA;
p21WAF1/CIP1;
acetylation
- From:
Chinese Pharmacological Bulletin
2017;33(10):1421-1425
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the regulation mechanisms of deacetylase inhibitor SAHA in p21WAF1/CIP1 promoter acetylation in breast cancer MCF-7 cells.Methods We used quantitative real-time PCR, Western blot and DNA-ChIP to determine the effects on the regulation of cell cycle with SAHA treatment in MCF-7 cells.By DNA-ChIP, we assessed the acetylation level of p21WAF1/CIP1 promoter.Results SAHA significantly affected the expression of cell cycle-related factors, and induced the mRNA and protein expression of p21WAF1/CIP1.SAHA could adjust the acetylation level of p21WAF1/CIP1 promoter.Conclusion SAHA regulates the cell cycle progression by adjusting the acetylation level of p21WAF1/CIP1 promoter in MCF-7 cells.
