3-D reconstruction of tetrachlorodibenzo-p-dioxin effected palatal organ development model of fetal mouse
10.3969/j.issn.1001-3733.2017.04.006
- VernacularTitle:三维重建观察四氯二苯并二恶英对胎鼠腭器官发育的影响
- Author:
Jixia CHAI
;
Dexiang SHEN
;
Xiaowan LIANG
;
Qing ZHOU
;
Zhicheng ZHANG
;
Jianhua KANG
;
Yiyi CHU
;
Qiang CHAI
- Keywords:
Tetrachlorodibenzo-p-dioxin (TCDD);
The palatal organ;
Three-dimensional reconstruction;
Lateral palatal process;
Development
- From:
Journal of Practical Stomatology
2017;33(4):455-458
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the feasibility of 3-D reconstruction model in the observation of tetrachlorodibenzo-p-dioxin(TCDD) effected palatal organ development of fetal mouse.Methods:Kunming mice treated 40 ug/kg TCDD by lavage on day 12.5 of pregnancy were used as in the experimental group,isodose corn oil treated in the control group.On day 13.5,14.5 and 15.5 of pregnancy heads of the fetal mice were taken out and fixed.Conventional paraffin serial sections of palatal organ were preparated and dyed by hematoxylin-eosin,images of the palatal organs were collected and photoshop treated,3-D reconstruction of the palatal organ was performed by 3D-DOCTOR software.Results:3-D reconstruction images showed that palatal organs moved from on both sides above the tongue and gradually closed and merged in the control group.In the experimental group,the palatal organs moved from on both sides above the tongue was later than control group,gradually closed,but not merged,formed cleft palate.Conclusion:3D-DOCTOR software reconstruction can be used for the study of the development process effected by TCDD in the pregnant mouse.
