The effects of HIF-1α on the growth of transplanted oral cancer in nude mice and on the expression of CEACAM1 and VEGF-C in the tumor
10.3969/j.issn.1001-3733.2017.04.007
- VernacularTitle:HIF-1α对口腔癌裸鼠移植瘤生长及其CEACAM1及VEGF-C表达的影响
- Author:
Yinhua DAI
;
Qun DAI
;
Wenying ZOU
;
Qiong YANG
;
Ziyan LIU
;
Yaoyu DONG
- Keywords:
Oral cancer;
Hypoxia inducible factor 1α(HIF-1α);
CEACAM1;
VEGF-C
- From:
Journal of Practical Stomatology
2017;33(4):459-463
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of HIF-1α on the growth of transplanted oral cancer and on the expression of CEACAM1 and VEGF-C in the tumor.Methods:Nude mouse model of oral cancer was established by transplantation of Tca8113 cells respectively treated by HIF-1α siRNA and negative control siRNA subcutaneously into right axillary region of nude mice.3 weeks after transplantation the mice were sacrificed,the tumor volum and weight were measured.The tumor tissue was examined by ELISA method for the detection HIF-1α protein expression,by real-time quantitative PCR and western blot for the detection of mRNA and protein expression of HIF-1α,CEACAM1 and VEGF-C respectively.Results:The volume and weight of the transplanted tumor in HIF-1 α siRNA group were significantly less than those in the control group(P＜0.05),CEACAM1 and VEGF-C mRNA and protein were down-regulate in HIF-1α siRNA group (P＜0.05).Conclusion:HIF-1α expression is positively related to the expression of CEACAM1 and VEGF-C in the regulation of oral tumor growth.
