The Modified Toluidine Blue Staining Method for Mast Cell

VernacularTitle:改良的肥大细胞甲苯胺蓝染色法
Author: Yuting LIANG ; Xia PENG ; Kun LIN ; Yue YIN ; Li LI
Keywords: Mast cell; Toluidine blue; Staining
From: Progress in Modern Biomedicine 2017;17(24):4601-4605
CountryChina
Language:Chinese
Abstract: Objective:This study aimed to identify the morphology of mast cells by using a modified toluidine blue staining scheme,so as to provide a powerful reference for the experimental basis research of mast cells.Methods:Bone marrow-derived mast cells were induced in vitro.After 4 weeks,the cells were collected,fixed,and stained.Mast cells were fixed at different temperature during different time.The optimum condition was determined by comparing the effects of toluidine blue staining.Results:Bone marrow cells were induced to differentiate into mast cells by SCF and IL-3 in vitro.When mast cells were stained with modified toluidine blue staining,the staining effect was better.Mast cells were round or oval and the cell membrane was complete and the cytoplasm was filled with a large number of purple particles.Conclusion:In this study,we successfully applied a modified toluidine blue staining method to mast cells cultured in vitro.The results showed that the condition at 37 ℃ full fixation with staining could reduce the degeneration of mast cells.This method was easy to operate with good stability.It was suitable for the morphological observation of mast cells cultured in vitro.