Effect of Ding's herb enema prescription on intestinal tissue relateddifferences target in rat colitis using protein array technology
10.3969/j.issn.1001-1978.2017.10.028
- VernacularTitle:基于蛋白芯片技术探讨丁氏溃结灌肠液对结肠炎大鼠肠道组织差异蛋白表达的影响
- Author:
Kang DING
;
Hui ZHANG
;
Yanyan TAN
;
Weina ZHU
;
Zhiwei LI
;
Wei LU
;
Ruiying LI
;
Min ZHAO
;
Yang DING
;
Meng LI
;
Shicai HUANG
;
Yijiang DING
;
Sumin ZHANG
- Keywords:
protein array;
Ding's herb enema prescription;
differences protein;
ulcerative colitis;
rat colitis induced by DSS;
intestinal fibrosis
- From:
Chinese Pharmacological Bulletin
2017;33(10):1473-1478
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of Ding''s herb enema prescription on intestinal tissue related target in rat colitis induced by dextran sulfate sodium(DSS), and to elucidate the mechanism of Ding''s herb enema prescription in improving the intestinal inflammation and intestinal fibrosis.Methods Rats were fed with 3.5％ DSS.The rats were randomly divided into positive drug group, model group, Control group, and Ding''s herb enema prescription group.The positive drug group was treated with mesalazine enema, and Ding''s herb enema prescription group was treated with Ding''s herb enema prescription.The colon mucosa was taken once a day for 6 weeks.The changes of intestinal inflammatory response and intestinal fibrosis related proteins were detected by GSR-CAA-67 antibody protein array, and the differentially expressed proteins were screened out.Results Eight proteins showed statistical differences, including IFN-γ, erythropoietin(EPO), TIMP-2, TIM-1, IL-6, TIMP-1, TNF-α, IL-22 (P<0.05).On the other hand, Ding''s herb enema prescription and mesalazine significantly antagonized the effect of IL-6 and TIMP-1 (P<0.05).The antagonized effect of Ding''s embolization enema on TNF-α and IL-22 was also significant(P<0.05), but mesalazine had no similar effect (P>0.05).Conclusions Ding''s herb enema prescription has the effect of multiple targets, which may improve the intestinal inflammatory response and intestinal fibrosis to achieve the purpose of treatment of ulcerative colitis(UC).
