Changes of MMP9 and TIMP 1 Expressions and Activity in the Carotid Artery of 4 wk Hindlimb Unweighted Rat
10.13241/j.cnki.pmb.2017.24.004
- VernacularTitle:四周尾部悬吊大鼠颈总动脉MMP9和TIMP1的表达及活性改变
- Author:
Xi ZHANG
;
Yuting SU
;
Yaoping CHENG
;
Xingxing MENG
;
Haijun ZHANG
;
Xiaoping XIE
;
Yaoming CHANG
;
Junxiang BAO
- Keywords:
Simulated weightlessness;
Rat;
Carotid artery;
Matrix metalloprotein9;
Tissue inhibitor of metalloproteinase1
- From:
Progress in Modern Biomedicine
2017;17(24):4615-4620,4667
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the changes of gene or protein expression and activity of matrix metalloprotein9 (MMP9) and tissue inhibitor ofmetalloproteinasel (TIMP1) in the carotid artery (CA) of 4 wk hindlimb unweighted rat.Methods:A 4 weeks(wk) hindlimb unweighted (HU) rat model was used to simulate the effect of weightlessness on the cardiovascular system.Transmission electron microscopy was used to detect the content of ECM.Reverse transcriptase polymerase chain reaction(RT-PCR) was conducted to measure the mRNA content MMP and TIMP1.Immunohistochemistry and Western blot technique were used to measure the protein abundance.Gelatin zymography was carried out to detect the activity of MMP9.Results:Compared to the control group (CON),the area of ECM was enhanced (P＜0.05) and the content of collage fiber was increased (P＜0.05) in the CA of HU rats;moreover,HU did not affect the mRNA expression of MMP9,but significantly reduced the protein content (P＜0.05) or enzymatic activity (P＜0.05).Accordingly,the mRNA or protein expression of TIMP1 in the CA was significantly increased by HU (P＜0.05).Conclusion:Simulated weightlessness caused imbalance between MMP and TIMP1 expression,which might contribute to the ECM aggregation and stiffness of CA.
