Observation on Microvascular Change of Bile Duct Ligation-induced Liver Fibrosis by X-ray Phase-contrast Computed Tomography
10.3969/j.issn.1005-5185.2017.03.002
- VernacularTitle:利用X射线相衬CT技术观察胆管结扎诱导肝纤维化微脉管的变化
- Author:
Lili QIN
;
Jianbo JIAN
;
Xinyan ZHAO
;
Chunhong HU
- Keywords:
Liver cirrhosis;
Tomography,X-ray computed;
X-ray diffraction;
Image processing,computer-assisted;
Pathology;
Disease models,animal;
Rats,Sprague-Dawley;
Male
- From:
Chinese Journal of Medical Imaging
2017;25(3):165-168,173
- CountryChina
- Language:Chinese
-
Abstract:
Purpose Liver fibrosis is the common pathological process of cirrhosis developed by chronic liver disease.This study aims to explore the application of microvascular change in assessing the liver fibrosis induced by bile duct ligation based on X-ray phasecontrast computed tomography (PCCT).Materials and Methods Ten rats were equally divided into the group of bile duct ligation-induced liver fibrosis and the control group at random.Then ten excised liver tissues were imaged at beamline BL13W1 of Shanghai Synchrotron Radiation Facility in China.After the imaging studies,the liver samples were stained with Sirius red and immunostained with antibodies against cytokeratin 19 (CK19).The microvasculature in livers was reconstructed via PCCT,and the correlation analysis on microvascular density and the proportion of fibrosis area was performed.Results The microvasculature including blood vessels,dilated bile ducts and ductular proliferation were clearly revealed by PCCT technology.Moreover,the 3D microvascular density and proportion of fibrosis area in the liver fibrosis group increased significantly compared with those in the control group (P＜0.01),and the correlation between them was obvious (r=0.812,P＜0.01).Conclusion Microvascular 3D characteristics of liver fibrosis are well demonstrated by PCCT,and 3D microvascular density is significantly correlated with the proportion of fibrosis area.Those findings indicate that microvascular change is closely related to the degree of liver fibrosis induced by bile duct ligation,and thus it provides a novel means to assess the severity of liver fibrosis through microvascular change.
