Effects of docosahexenoic acid on large conductance Ca+-activated K+ channels in retinal smooth muscle cells
10.3760/cma.j.issn.1005-1015.2017.03.017
- VernacularTitle:二十二碳六烯酸对视网膜动脉平滑肌细胞大电导钙激活钾离子通道的激活作用
- Author:
Xuan CHEN
;
Jun SHAO
;
Dayun XIA
;
Ruxing WANG
;
Yong YAO
- Keywords:
Docosahexaenoic acids;
Retinal artery;
Myocytes,smooth muscle;
Large-conductance calcium-activated potassium channels;
Patch-clamp techniques
- From:
Chinese Journal of Ocular Fundus Diseases
2017;33(3):295-297
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects ofdocosahexenoic acid (DHA) on large conductance Ca2+-activated K+ (BK) channels in normal retinal artery smooth muscle cells (RASMCs).Methods Cultured human RASMCs (6 th-8 th generations) were used to patch clamp experiment.The open probabihties (NP0) in BK channels with different concentrations (0.0,1.0,3.0,5.0,7.5,10.0 μmol/L) of DHA were recorded by patch clamp technique in single channel configuration.RASMCs were intervened by different concentrations (0.0,1.0,5.0 μmol/L) of DHA as control group,low and high doses of DHA groups,respectively.The protein expressions of β subunit of BK channels in RASMCs from three groups were measured by Western blot.Results The NP0 of BK channels were 0.044 4±0.001 2,0.081 2±0.004 2,0.209 0±0.006 1,0.310 5±0.005 3,0.465 0±0.007 8 and 0.497 7±0.014 5 with perfusate of 0.0,1.0,3.0,5.0,7.5,10.0 μtmol/L DHA.DHA activated BK channels in a dose-dependent manner (F=2.621,P＜0.05).There was no significant difference in the protein expression of control group,low and high doses of DHA groups (F=1 1.657,P＞0.05).Conclusion DHA can directly activate BK channels,no increasing in subunit expression of BK channels.
