Regulating effects of Dickkopf-1 on Wnt signaling pathway at differentiation of osteoblast in rat

VernacularTitle:Dickkopf-1在大鼠成骨细胞分化过程对Wnt信号途径的调控效应
Author: Xiuyang WAN ; Shoufang SUN ; Yonghui LIU ; Nian DENG ; Zhijun XING ; Hongyan YUAN ; Liang ZHANG
Keywords: Osteoblasts; Bone remodeling; DKK-1; Wnt pathway
From: Chinese Journal of Primary Medicine and Pharmacy 2017;24(18):2838-2841,后插2
CountryChina
Language:Chinese
Abstract: Objective To discuss the effect of Dickkopf-1 (DKK-1) on Wnt singal pathway during the differentiation of osteoblast in vitro.Methods The osteoblasts were obtained from the new rats and cultured in vitro.The 3 passages were divided into control group and DKK-1 group.The cells were cultured in DKK-1 and normal saline for morphogical detection,ALP activity detection and osteoblasts stained at 1 st,6th,12th,21st day.The Wnt was detected by RT-PCR.Results After cultured by the DKK-1 in vitro,the ALP and mineralization of osterblasts staining were prlonged with culture time.Compared with control group,the expression of Wnt was significantly reduced at the 21st day after induction (t =0.278,P ＜ 0.05).Conclusion DKK-1 can regulate the expression of Wnt during osteoblast differentiation,suggests that Wnt may be involved in osteoblast differentiation and can affect bone remodeling process.