Comparison of the minimum inactivated time between the Nakayama and Beijing-1 strain of Japanese encephalitis (JE) virus
- Author:
Tran Hang Nga
- Publication Type:Journal Article
- Keywords:
Japanese encephalitis
- MeSH:
Encephalitis, Japanese;
Time Factors
- From:Journal of Practical Medicine
2005;515(7):58-59
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
The strains of JE vaccine were produced from Nakayama strain: S461, S462, S463 and from Beijing-1 strain: BS0704, BS0804, BS0904. The content of formalin for inactivation was 0.06% at 40C. The schedule for testing: 0, 3, 7, 14, 21, 28 and 35 days. The crude virus was inoculated directly into IC 0.03 ml/mouse then followed up within 14 days. Calculated LD50 according to Reed and Muench method. Results: LD50 decreased step by step and LD50 = 0 at the 28th days. That means from 28th days, crude vaccine which was produced from Nakayama and Beijing-1 strains were completely inactivated.
