Scaffolds, cells and cytokines: vascularization strategies for tissue engineering
10.3969/j.issn.2095-4344.2017.14.021
- VernacularTitle:支架、细胞、因子:组织工程血管化策略的研究与前景
- Author:
Haiyan LI
;
Liu ZOU
;
Lixian SHEN
;
Bin ZHOU
;
Ping ZHANG
;
Yu GUO
- From:
Chinese Journal of Tissue Engineering Research
2017;21(14):2259-2265
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Tissue-engineered skin, bone, cartilage, blood vessel, and muscle flap have achieved remarkable development in tissue repair and regeneration; meanwhile, the difficulty in tissue-engineered vascularization has attracted much attention.OBJECTIVE: To summarize studies concerning the mechanism of angiogenesis (vasculogenesis and revascularization),vascularization strategies (seed cells, scaffolds, growth factors) and models of vascularization in tissue engineering (in vivo and in vitro), and to provide theoretical foundation for basic research of vascularization in tissue engineering.METHODS: A computer-based research was performed for relevant literatures in PubMed, Springerlink, Web of Science,ScienceDirect, CNKI, CqVip and WanFang databases published between January 20002 and April 2016 using the keywords of issue engineering, vascularization, scaffolds, cell growth factor, vasculogenesis, angiogenesis in English and Chinese, respectively. Fifty-five eligible articles were included according to the inclusion and exclusion criteria.RESULTS AND CONCLUSION: In vitro microvascular construction is commonly used. The angioid scaffold is prepared,and then cells are seeded onto the scaffold, which will proliferate, migrate and form microvessels induced by growth factors. The cell-scaffold composites that have good vascularization in vitro have been introduced via transplantation in in vivo experiments. However, there are some problems, such as the inevitable inflammatory reaction that makes a direct effect on the composite function and survival, and vascularization is still a difficulty in tissue regeneration and repair in the tissue engineering research.
