Determination of Copper Ion in Rabbit with Implantation of Copper Intrauterine Device
10.3870/j.issn.1004-0781.2017.06.006
- VernacularTitle:含铜节育器植入家兔体内血清铜离子含量测定
- Author:
Xiaoling XU
;
Kaiyong HE
;
Wangping PAN
;
Xiaojun LYU
;
Yuanhua HU
- Keywords:
Copper intrauterine device;
Copper ion;
Inductively coupled plasma mass spectrometry
- From:
Herald of Medicine
2017;36(6):614-616
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a method for determination of serumal copper ion in rabbit with implantation of copper intrauterine device.Methods At different time points after implantation,the serumal copper ion concentrations were determined by microwave digestion-inductively coupled plasma mass spectrometry(ICP-MS) using scandium(Sc) and indium(In) as internal standards to compensate sample matrix effects,and the pharmacokinetics parameters were calculated in order to reflect copper ion release and metabolic rule.Results The serumal copper ion concentrations were kept at a low and stable level.The recoveries were in the range of 98.7%-113.3%,with the relative standard deviations of less than 5.0%.Conclusion The analytical method is simple,fast,and can be used for the determination of serumal copper ion in rabbit.
