Analysis of Triterpenoids Component in Loquat Leaf Herbs and Its Preparations by HPLC

VernacularTitle:高效液相色谱法测定枇杷叶及其制剂中三萜类成分
Author: Shita ZHU ; Fengling ZHANG ; Ping JIN ; Yao XIAO
Keywords: Preparations of Loquat leaf; Ursolic acid; Oleanolic acid; Chromatogram; high performance liquid
From: Herald of Medicine 2017;36(6):673-676
CountryChina
Language:Chinese
Abstract: Objective To determine the triterpenoid content in Loquat leaf by using HPLC method and to explore the feasibility of ursolic acid and oleanolic acid as the quantitative markers for determination of Loquat leaf and its preparations.Methods Two desugaring methods of Loquat leaf extracts were compared.The contents of ursolic acid and oleanolic acid in Loquat leaf as well as preparations were measured based on a system of HPLC,with a Dumas Diamonsil C18 column (250 mm×4.6 mm,5 μm) was used as analytical column.The mobile phase was consisted of acetonitrile-0.1% phosphoric acid (95∶5) with the flow rate of 0.5 mL·min-1.The column temperature was 30 ℃,the injection volume was 10 μL, the detective wavelength was 210 nm.Results Ursolic acid and oleanolic acid were measured in both Loquat leaf and its preparations,and their contents were fairy stable and comparable.Conclusion Ursolic acid and oleanolic acid can be served as quantitative markers for the determination of Loquat leaf and its preparations.