In Vivo Intestinal Absorption Characteristics of Dioscin from Dioscorea nipponica Makino Extract in Rat
10.3870/j.issn.1004-0781.2017.06.005
- VernacularTitle:穿山龙提取物中薯蓣皂苷的大鼠在体肠吸收
- Author:
Xihui BAI
;
Shiyu LIU
;
Jing WANG
;
Changli WANG
;
Xiao SONG
- Keywords:
Dioscorea nipponica Makino extract;
Dioscin;
Single-pass intestine perfusion model;
P-glycoprotein
- From:
Herald of Medicine
2017;36(6):610-613
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study absorption characteristic of dioscin from Dioscorea nipponica Makino extract in rat intestine.Methods Single-pass intestinal perfusion (SPIP) model was used for rat in situ and HPLC was used to determine the concentrations of dioscin.The effects of different intestinal segments,drug concentration and P-glycoprotein (P-gp) inhibitor on intestinal absorption were investigated.Results Dioscin could be absorbed in the whole intestine,the absorption rate constant (Ka) and the apparent coefficient (Papp) of dioscin decreased following the sequence of ileum > duodenum =jejunum > colon.Absorption parameters of dioscin had no significant difference at different concentrations (40,80,120 mg·L-1).There were significant differences in Ka and Papp values between P-gp inhibitor group and no P-gp inhibitor group(P<0.05).Conclusion The saturate phenomena was not observed under the test range of drug concentration,and the absorption mechanism may be passive diffusion transport.Dioscin in Dioscorea nipponica Makino extract may be the substrate of P-gp.
