Salidroside regulates Akt/GSK-3β/CRMP-2expression and axonal regeneration in MCAO rats
10.3969/j.issn.1001-1978.2017.09.025
- VernacularTitle:红景天苷对局灶性脑缺血/再灌注大鼠轴突再生及Akt/GSK-3β/CRMP-2表达的影响
- Author:
Xiaoqin ZHANG
;
Xuezheng MIAO
;
Liying ZHOU
;
Liwei XU
;
Wenfang LAI
;
Guizhu HONG
- Keywords:
salidroside;
local cerebral ischemia/ reperfusion;
axonal regeneration;
Akt;
GSK-3β;
CRMP-2
- From:
Chinese Pharmacological Bulletin
2017;33(9):1320-1324
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the axonal regeneration effect of salidroside in MCAO rats and its potential mechanism.Methods Thirty-six healthy adult male Sprague-Dawley rats were randomly divided into three groups: sham, MCAO, MCAO+Sal groups.The rats were subjected to focal cerebral ischemia/reperfusion with suture-occluded method.Neurological deficit testing was performed with Zea Longa scale.The protein expression of p-Akt(Ser473), Akt, p-GSK-3β(Ser9), GSK-3β, p-CRMP-2(Thr514) and CRMP-2 in side cerebral ischemic tissues were determined using Western blot analysis.NF200 immunofluorescence staining was used to evaluate axonal regeneration.Results Compared with MCAO group,salidroside significantly improved the neurological deficit,up-regulated the protein expression of NF200,p-Akt and p-GSK-3β,and inhibited the protein expression of p-CRMP-2.Conclusions Salidroside improves neurological function recovery after focal cerebral/ischemic injury in rats,which may be associated with the up-regulation of phosphorylated Akt and GSK-3β and inhibition of phosphorylated CRMP-2,thereby promoting axonal regeneration.
