Effects of Dredging Collaterals and Activating Blood Worm Chinese Materia Medica on Angiogenesis Related Factors of Lung Cancer in Hypoxic Environment
10.3969/j.issn.1005-5304.2017.09.010
- VernacularTitle:通络活血虫类药对乏氧环境下肺癌血管生成相关细胞因子的影响
- Author:
Daorui LI
;
Miaomiao WANG
;
Mingwei YU
;
Fei LIN
;
Ying ZHANG
;
Meng LI
;
Huiting FAN
;
Qi ZHENG
;
Xin QI
;
Yingxia PEI
;
Peitong ZHANG
;
Wei HOU
;
Hongsheng LIN
- Keywords:
dredging collaterals and activating blood;
worm Chinese materia medica;
lung cancer;
hypoxia
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(9):39-42
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of dredging collaterals and activating blood worm Chinese materia medica on angiogenesis related factors of lung cancer in hypoxic environment. Methods The lung cancer A549 cells were cultured in vitro to simulate tumor hypoxia microenvironment by the hypoxia workstation, and different concentrations of Scorpio, Scolopendra and Gecko medicated serum were added. MTT method was used to detect cell proliferation and screen the best medicine concentration and duration of action. Lung cancer A549 cells were administrated by the three kinds of medicated serum, and cells were collected and supernatant was cultured. Contents of VEGF, TGF-β1, and bFGF were detected by ELISA. Results Three kinds of medicated serum had the inhibitory effect on both added normoxia and hypoxia in cultured A549 lung cancer cells. 7.5% concentration of medicated serum was selected, and 24 h later were used in later experiments. Scorpio, Scolopendra and Gecko medicated serum can more reduce the contents of VEGF, TGF-β1 and bFGF in the supernatant of A549 cell compared with the control group (P<0.05, P<0.01). Conclusion Dredging collaterals and activating blood worm Chinese materia medica had inhibitory effect on cancer cells and the regulation of angiogenesis related cytokines in the condition of normoxia and hypoxia.
