Content Determination of Gallic Acid, Corilagin and Ellagic Acid in Aqueous Extract from Sanguo Decoction by HPLC

VernacularTitle:HPLC同时测定三果汤水提物中没食子酸、柯里拉京及鞣花酸含量
Author: Bin LI ; Xin LI ; Yuan FAN
Keywords: aqueous extract from Sanguo Decoction; HPLC; gallic acid; corilagin; ellagic acid
From: Chinese Journal of Information on Traditional Chinese Medicine 2017;24(9):76-79
CountryChina
Language:Chinese
Abstract: Objective To establish an HPLC method for the simultaneous determination of contents of gallic acid, corilagin and ellagic acid in aqueous extract from Sanguo Decoction. Methods The analysis was carried out on an Agilent C18 column (4.6 mm × 250 mm, 5 μm), and the mobile phase was composed of acetonitrile and 0.1%phosphoric acid aqueous with gradient elution. The detection wavelength was 260 nm. The flow rate was 1.0 mL/min at column temperature of 30 ℃. Results Gallic acid, corilagin and ellagic acid were completely separated and the peak shape was good. It showed a good linearity in the concentration range of 0.174–2.61 μg, 0.04–0.60 μg, and 0.052–0.78 μg, respectively. The average recoveries of gallic acid, corilagin and ellagic acid were 99.98%, 99.81%and 100.12%, respectively. The RSD were less than 2.0%. Conclusion This method is rapid, accurate and repeatable, which can provide references for the quality control of Sanguo Decoction.