Effect and mechanism of aspirin to inhibit metastasis of colorectal cancer in mice
10.11659/jjssx.12E016088
- VernacularTitle:阿司匹林对抑制结肠癌小鼠肝转移的作用及机制研究
- Author:
Wen YUAN
;
Qiujuan CHEN
;
Xin LIU
;
Zihao YANG
;
Ping LIU
;
Fangyi LONG
;
Ting WANG
- Keywords:
colorectal cancer;
liver metastases;
aspirin;
vascular endothelial growth factor
- From:
Journal of Regional Anatomy and Operative Surgery
2017;26(5):319-323
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect of aspirin on liver metastasis of colon cancer in mice and the possible mechanism.Methods A total of 32 BALB/C mice were injected with CT26 colorectal cancer cells to establish colon cancer liver metastatic model,with 3 mice dead,15 mice in control group and 14 mice in experimental group.The control group was given saline 0.2 mL/d,the experimental group were given aspirin 30 mg/kg.The liver weight and the number of metastatic tumors were calculated after 30 days of intervention.HE and CD31 staining was performed by immunohistochemistry to observe the metastasis and angiogenesis.The protein expression of VEGF and cox-2 were analyzed by Western blot.Results The average liver weight and number of liver metastases nodules in the experimental group were significantly lower than those in the control group(P<0.05).Pathological examination showed that the experimental group of mice the number of liver cells and liver tumor angiogenesis were significantly less than the control group(P<0.05).Western blot showed that the expression of VEGF and cox-2 of CT26 cells were down-regulated after treated with aspirin.Conclusion Aspirin can down regulate the protein expression of VEGF and cox-2 protein to inhibit liver metastasis of colon tumor proliferation and angiogenesis,thereby inhibiting metastasis of colon cancer cells,for therapeutic purposes.
