Impact of Insulin Resistance and Adiponectin Lacking on Myocardial Remodeling in Experimental Mice
10.3969/j.issn.1000-3614.2017.08.018
- VernacularTitle:胰岛素抵抗及脂联素缺乏对小鼠心肌重构的影响
- Author:
Lu XU
;
Yajuan LIU
;
Qing ZHANG
;
Yi QIN
;
Na ZHANG
;
Yongkang REN
;
Ruiying YANG
- Keywords:
Insulin resistance;
Adiponectin;
Ventricular remodeling
- From:
Chinese Circulation Journal
2017;32(8):808-812
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the impact of insulin resistance (IR) and adiponectin (APN) lacking on myocardial remodeling in experimental mice. Methods:16 normal C57 mice were divided into 2 groups: Control group and IR group; in addition, 16 APN gene knockout(APNKO) mice were divided into another 2 groups: APNKO group and APNKO+IR group.n=8 in each group. The mice in Control group and APNKO group were fed with normal diet, in IR group and APNKO+IR group were fed with high fat diet to create the IR model. All animals were treated for 12 weeks. Blood levels of total cholesterol(TC), triglycerides(TG),fasting plasma glucose(FPG) and fasting insulin(FINS) were examined; heart weight and left ventricular weight were measured; left ventricular myocardial morphological changes and the degree of ifbrosis were assessed by HE staining and Masson staining; protein expressions of myocardial matrix metalloproteinase-9 (MMP-9) and APN were detected by immunehistochemistry and Western blot analysis. Results: Compared with Control group, IR group, APNKO group and APNKO+IR group showed elevated blood levels of TC, TG, FPG and FINS; increased heart weight and left ventricular weight, allP<0.05; IR group, APNKO group and APNKO+IR group presented more myocardial hypertrophy, decreased protein expression of APN and increased protein expression of MMP-9, allP<0.05.Conclusion: IR and APN lacking could incur myocardial remodeling in experimental mice and they had synergistically facilitated effect.
