The mitochondrial toxicity of bentysrepinine on HepG2 cells
10.3969/j.issn.1001-1978.2017.09.013
- VernacularTitle:替芬泰对HepG2细胞的线粒体毒性
- Author:
Yue FENG
;
Xuan HUO
;
Jinfang HU
;
Zhiquan DI
;
Zongpeng ZHANG
;
Xiuping SHEN
- Keywords:
bentysrepinine;
mitochondrial toxicity;
MMP;
ROS;
mitochondrial respiratory chain complex enzymes;
lactic acid
- From:
Chinese Pharmacological Bulletin
2017;33(9):1248-1252
- CountryChina
- Language:Chinese
-
Abstract:
Aim To provide references for clinical trials dose and rational drug use by evaluating mitochondrial toxicity of bentysrepinine on HepG2 cells.Methods Mitochondrial toxicity of bentysrepinine on HepG2 cells was cmomprehensively evaluated by measuring proliferation inhibition rate, lactic acid content in culture supernatant, reactive oxygen species(ROS) content, mitochondrial membrane potential (MMP) variation and the activity of mitochondrial respiratory chain complex enzymes Ⅰ to Ⅳ.Results The half inhibitory concentration of bentysrepinine of HepG2 cells was 359 μmol·L-1.Compared with the control group, bentysrepinine could reduce the MMP, raise the level of lactic acid, increase the content of ROS and lower the activity of mitochondrial respiratory chain complex enzymes Ⅰ to Ⅲ with the concentration of 400 μmol·L-1(196 mg·L-1), showing an obvious mitochondrial toxicity.Compared with lamivudine and adefovir dipivoxil, bentysrepinine exerted no influence on indexes above with the same concentration 100 μmol·L-1.Conclusions Bentysrepinine shows an obvious mitochondrial toxicity on HepG2 cells with the concentration of 400 μmol·L-1.This mitochondrial toxicity is not presented with the concentration of 200 μmol·L-1.It shows that the safety range of bentysrepinine about mitochondrial toxicity is relatively wide.The test plays a guiding role in clinical trial dose design as well as clinical treatment.
