Effect of curcumin in prolonging survival timeof cryopreserved mice via promoting thyroid function
10.3969/j.issn.1001-1978.2017.09.020
- VernacularTitle:基于促甲状腺功能的姜黄素延长低温冷冻小鼠生存时间的作用机制研究
- Author:
Zhaoxue LI
;
Siyuan HU
;
Jiye WANG
;
Zehui CHEN
;
Shanshan ZHANG
;
Bo ZHANG
- Keywords:
curcumin;
cold injury;
thyroid;
survival time;
Kunming mice;
propylthiouracil;
sodium levothyroxine
- From:
Chinese Pharmacological Bulletin
2017;33(9):1291-1297
- CountryChina
- Language:Chinese
-
Abstract:
Aim To look for cold-protective drugs treating cryogenic freezing that may bring great damage to animal physiological system.Methods The protective effect of curcumin on frozen damage and the changes of thyroid function in mice were studied in this study.Quantitative analysis of the changes in survival time and metabolic indexes in mice disposed at(-20±1)℃ was conducted.Mouse serum free triiodothyronine(FT3) and free thyroxine(FT4) contents were detected by ELISA kit.HE staining was used to observe thyroid tissue morphological items.The expression of genes related to thyroid function was assessed via real-time quantitative PCR.Results The intraperitoneal injection of curcumin(12.5~50 mg·kg-1) could remarkably prolong the survival time of mice when exposed to cryogenic freezing.HE staining results displayed a recovered thyroid injury in morphology in the curcumin group, further with a notably improved metabolic indexes and evidently increase in serum FT3 and FT4 levels.The real-time quantitative PCR results indicated that the expressions of sodium iodide symporter(Nis), thyroglobulin(Tg) and thyroid peroxidase(Tpo) were up-regulated, and the expression of thyroid-stimulating hormone receptor(Tshr) was down-regulated.Sodium levothyroxine collabrated with the promoting thyroid effects of curcumin, while propylthiouracil inhibited the effects.Conclusion Curcumin can prolong the survival time of the cryogenic freezing mice, which is closely related to its ability to promote thyroid function.
