Effects of Zuogui Jiangtang Jieyu Prescription on Neurotrophic Effects of Astrocytes in Diabetes Mellitus Rats with Depression

Hui Yang; Yuhong Wang; Qing DU; Yuanshan Han; Pan Meng; Jian Liu; Lin Liu; Hongqing Zhao

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Effects of Zuogui Jiangtang Jieyu Prescription on Neurotrophic Effects of Astrocytes in Diabetes Mellitus Rats with Depression

VernacularTitle:左归降糖解郁方对糖尿病并发抑郁症大鼠海马星形胶质细胞神经营养的影响
Author: Hui YANG ; Yuhong WANG ; Qing DU ; Yuanshan HAN ; Pan MENG ; Jian LIU ; Lin LIU ; Hongqing ZHAO
Keywords: Zuogui Jiangtang Jieyu Prescription; diabetes mellitus with depression; astrocyte; neurotrophy; rats
From: Chinese Journal of Information on Traditional Chinese Medicine 2017;24(9):43-47
CountryChina
Language:Chinese
Abstract: Objective To investigate the effects of Zuogui Jiangtang Jieyu Prescription on neurotrophic effects of astrocytes in diabetes mellitus rats with depression. Methods The diabetes mellitus with depression rat models were established by composite method, and then were randomly divided into 5 groups: model group, positive group, Zuogui Jiangtang Jieyu Prescription high-, medium-, low-dose groups, with 12 rats in each group. 12 normal rats were set as control group. Each administration group was given relevenat medicine for gavage for continuous 4 weeks. Open-field test was used to evaluate the depression-like behavior. The expression of GFAP in astrocyte and MAP2 in neuron were tested by immunohistochemistry. The expression of protein and mRNA of BDNF, GDNF, and NGF were tested by Western blot and RT-PCR. Results Compared with the control group, the activities of rats in model group were significantly reduced. The expression of GFAP increased, while the expressions of MAP2, BDNF, GDNF and NGF decreased. Compared with the model group, the depression-like behavior of rats in model group were significantly improved. The expression of GFAP decreased, while the expressions of MAP2, BDNF, GDNF and NGF increased, and GFAP decrseaed significantly. Conclusion The secretion function of astrocyte can be improved by Zuogui Jiangtang Jieyu Prescription. Its anti-depression and neuron-protection function might be correlated with the enhancement of neurotrophic effects of astrocytes.