Research on Separation and Purification of Extract from Hyssopus cuspidatus Boriss. with Polyamide Resins
10.3969/j.issn.1005-5304.2017.09.016
- VernacularTitle:聚酰胺树脂分离纯化神香草提取液工艺研究
- Author:
Yan MAO
;
Jinhua HE
;
Xintang WANG
;
Xiaojuan RONG
- Keywords:
Hyssopus cuspidatus Boriss.;
polyamide resins;
total flavonoid;
rosmarinic acid;
purification process
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(9):63-67
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the optimal process conditions for the separation and purification of extract from Hyssopus cuspidatus Boriss. by polyamide resins. Methods The total flavonoids and rosmarinic acid were used as the indexes. The maximum amount of sample solution, elution volume, concentration of sample solution, adsorption time of resin, loading time of sample solution and the amount of eluting solvent, pH and elution rate in the resin purification process were screened by single factor method. Results The optimal purification parameters were as follows: 10 mg/mL of extract, 12 mL of sample amount, 2 BV of water to remove impurities, 40% ethanol to elute 9 BV; the concentration of rosmarinic acid in sample solution was 86.3 μg/mL, and the total flavonoid concentration was 117.8 μg/mL; the resin adsorption time was 14 h; the pH of sample solution was 6.5; the elution rate was 3.0 BV/h. Conclusion This method is simple and feasible, fit for separating and purifying of extract from Hyssopus cuspidatus Boriss.
