Content Determination of Phellodendrine in Leshu Lotion by SPE-HPLC
10.3969/j.issn.1005-5304.2017.09.018
- VernacularTitle:SPE-HPLC测定乐舒洗液中黄柏碱含量
- Author:
Ni YANG
;
Guifu MAO
;
Honghong WANG
;
Wei XIE
;
Wei LIN
- Keywords:
solid phase extraction;
HPLC;
Leshu Lotion;
phellodendrine
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(9):73-75
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the method of thin layer identification of Phellodendri Chinensis Cortex and solid phase extraction - high performance liquid chromatography (SPE-HPLC) determination of the content of phellodendrine in Leshu Lotion. Methods Thin layer chromatography (TLC) was used for qualitative identification of Phellodendri Chinensis Cortex in Leshu Lotion and the Bond Elutplexa PCX strong cation-exchange solid phase was used for extraction small column purification. Platisil-NH2 column was as chromatographic column; column temperature was 30 ℃; triethylamine pH 3.82 (phosphoric acid), tetrahydrofuran, acetonitrile was 18:12:70, isocratic elution; flow rate was 1.0 mL/min; detection wavelength was 286 nm. Results TLC could obviously identify active ingredients of phellodendrine in Phellodendri Chinensis Cortex. The phellodendrine had good linear relationship between concentration and peak area within the scope of 1.22–12.2 μg (r=0.9999); the average recovery rate of phellodendrine was 101.18%, RSD was 1.71%. Conclusion This method is simple to operate, with accuracy and repeatability, and can be used for quality control of Leshu Lotion.
