Content Determination of Phenylethanoid Glycosides and Acteoside in Plantago Herba from Different Producing Areas
10.3969/j.issn.1005-5304.2017.09.021
- VernacularTitle:不同产地车前草中总苯乙醇苷和毛蕊花糖苷含量测定
- Author:
Man YI
;
Chuanhua FENG
;
Xiaolin TANG
;
Lan XU
;
Xiaoxuan TAO
;
Lang ZHANG
;
Gang LI
- Keywords:
Plantago Herba;
phenylethanoid glycosides;
acteoside
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(9):84-86
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determination of phenylethanoid glycoside and acteoside in Plantago Herba. Methods UV-visible spectrophotometric method was used for the determination of the content of phenylethanoid glycosides compounds in Plantago Herba. HPLC method was used for the determination of acteoside in Plantago Herba. Chromatographic column with C18 ODS2 (4.6 mm × 150 mm, 5 μm) was used. Acetonitrile-0.1%formic acid (13:87) was as mobile phase; the flow rate was 1 mL/min; the detection wavelength was 332 nm; the column temperature was 30 ℃; the sample volume was 10 μL. Results The contents of phenylethanoid glycoside in Plantago Herba from different producing areas were among 1.03%–3.47%. Acteoside with peak area over the 0.0062–1.55 mg range showed a good linear relationship; the sample recovery rate was 98.9%, and the RSD was 1.6%. The contents of acteoside in Plantago Herba from different producing areas was among 0.18%–0.56%. Conclusion The method is simple, stable and reproducible, which can be used for the determination of phenylethanoid glycoside and acteoside in Plantago Herba from different producing areas and provide experimental basis for quality control of Plantago Herba.
